Application

The pH values of all buffers are temperature and concentration dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.

General description

Useful in the pH range of 7.0-9.0. Has a pK_a of 8.1 at 25°C.

Absorbance (1.0 M, H₂O, 260 nm):≤0.1.

Widely used as a biological buffer (absorbance: ﹤0.1 at 260 nm, 1.0 M, H₂O). A 1.0 M solution in H₂O has a pH range of 3.5-5.0 and a pK_a of 8.3 at 20°C.

Legal Information

ULTROL is a registered trademark of Merck KGaA, Darmstadt, Germany

Trizma is a registered trademark of Sigma-Aldrich Co. LLC

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Packaging

250, 500 g in Glass bottle

1 kg in Glass bottle

Warning

Toxicity: Standard Handling (A)